Time estimate (each level is a single segment):

Assault On FCB (01:08.80)
Freeport City Bank (01:19.37)
Abandoned Buildings (00:59.13)
Construction Zone (01:38.87)
Abandoned Subway (02:48.13)
Sintek Chemical Lab (00:50.07)
Sintek Chemical Plant (02:22.67)
Sintek Warehouse 1 (01:25.73)
Sintek Warehouse 2 (01:19.80)
Biomech Factory 1 (01:59.37)
Biomech Factory 2 (02:17.70)
Freeport Sewers (00:45.33)
Lower Freeport Sewers (00:45.83)
Freeport Dam (00:56.73)
Sintek Oilrig (02:32.77)
Underwater Passage 1 (03:01.30)
Underwater Passage 2 (02:48.50)
Hidden Docks 1 (01:00.37)
Hidden Docks 2 (00:44.50)
Jungle Pass 1 (00:28.10)
Mountain Gorge (01:14.23)
Containment Area 57 (02:07.70)
Xenomorphic Lab 1 (00:32.13)
Xenomorphic Lab 2 (00:39.00)
Estate Sinclaire 1 (00:17.63)
Estate Sinclaire 2 (00:28.33)
Munt Phoenix (01:29.10)
Thrall Master (03:57.67)

Total: 41:58.76