Estimate for time is 41:59. I think time should start at the click of start game and end at the prompt to overwrite previous endgame save data. It is a single segment run. The long load times are not due to my computer but rather a painful quirk of the game itself.

RNG was kind, I only had one random encounter on the map on the battle with the Captain went faster than normal. The one death I had wasn't quite ideal but still despawns the enemy and it would have been slower to fight him before initially taking the raft and fighting the captain/slaver.